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On of MAGE-A1 expressionIn order to functionally investigate the impact of BORIS on promoter settings, we examined the influence of BORIS on the activity of the methylated MAGE-A1 promoter in the context of transcription factors Ets-1 and Sp1. We transiently co-transfected methylated reporter plasmids pGL2/MAGE-A1 (-77/+183) containing the BORIS binding site located downstream of the start site (F
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